(Jocks and Roessner 2009/2010)ĭLS is extremely sensitive to the presence of aggregates as the molecular weight dependence of the particle scattering intensity. Note the perfect well-to-well reproducibility of the measurements. Protein measurement using the Plate Reader: Comparison of sources A and B in pH 5 buffer. Discovery of aggregate-based inhibitors represents a new tool for drug discovery.įigure 1. This aggregation happens under suitable bioassay buffer conditions, and then non-specifically inhibits receptors and enzymes, producing a false-positive result. One false-positives mechanism refers to aggregate-based inhibitors. However, a high throughput of automation does not guarantee a more fruitful drug discovery and development process due to false-positives encountered during the screening of compound libraries. High throughput combinatorial techniques are often used for new drugs discovery. Particle concentration, type of ions in the medium can also be detected. We can measure amounts of large particles (aggregates) not detected by other means.ĭLS has several applications to support drug discovery process:ĭLS can be used to characterize the size of various particles including proteins and small compounds. Our platform facilitates formerly laborious and time-consuming measurements, such as aggregates screening or optimal crystallization conditions determination. This non-invasive technique yields valuable information of drug leads. Our novel DLS plate reader has incomparable advantages which require as little as 50 microliter sample volumes, utilizing disposable microplates (up to 1536 samples), and detecting chemical aggregates at compound concentrations in the range of 3 to 10°M. In addition to measuring small molecules, DLS can also be used to probe the behavior of complex fluids, such as concentrated polymer solutions.Ĭreative Biolabs has a world-class DLS system for measuring the effective size of molecules. Elaborated multi-angle instruments can determine the full particle size distribution. The sample is illuminated by a laser beam and the temporal fluctuations are analyzed at a known scattering angle θ by means of the intensity or photon autocorrelation function. Creative Biolabs provides automated DLS technology to aid your drug discovery process.ĭLS is a physical technique, which is capable of determining the particle size of very small particles and polymers in solution, even at low sample concentrations. This popular technique allows particle sizing down to 1 nm in diameter. Pharmacology and Pharmacodynamics Studiesĭynamic light scattering (DLS) or photon correlation spectroscopy is one of the most popular light scattering techniques that measuring the particle size distribution.Developability Assessment of Biopharmaceutical Candidates.Small-Angle X-ray and Neutron Scattering.Nuclear Magnetic Resonance (NMR) Spectroscopy.Natural Bioactive Compound Target Identification.In Silico Functional Analysis for Fungi.Computer-Aided Target Identification and Validation.Identification of Biomarkers by ORFeome Phage Display.Target Discovery for Cancer Immunotherapy.
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